Jane Eyre is divided into 38 chapters and most editions are at least 400 pages long (although the preface and introduction on certain copies are liable to take up another 100 or so). The original was published in three volumes, comprising chapters 1 to 15, 16 to 26, and 27 to 38; this was a common publishing format during the 19th century, see Three-volume novel.
Brontë dedicated the novel's second edition to William Makepeace Thackeray.
The novel begins with a ten-year-old orphan named Jane Eyre who is living with her uncle's family, the Reeds, as her uncle's dying wish. Jane's parents died of typhus. Jane’s aunt Sarah Reed does not like her and treats her like a servant. She and her three children are abusive to Jane, physically and emotionally. One day Jane gets locked in the room in which her uncle died, and panics after seeing visions of him. She is finally rescued when she is allowed to attend Lowood School for Girls.
Jane arrives at Lowood Institution, a charity school, with the accusation that she is deceitful. During an inspection, Jane accidentally breaks her slate, and Mr. Brocklehurst, the self-righteous clergyman who runs the school, brands her as a liar and shames her before the entire assembly. Jane is comforted by her friend, Helen Burns. Miss Temple, a caring teacher, facilitates Jane's self-defense and writes to Mr. Lloyd whose reply agrees with Jane's. Ultimately, Jane is publicly cleared of Mr. Brocklehurst's accusations.
The eighty pupils at Lowood are subjected to cold rooms, poor meals, and thin clothing. Many students fall ill when a typhus epidemic strikes. Jane's friend Helen dies of consumption in her arms. When Mr. Brocklehurst's neglect and dishonesty are discovered, several benefactors erect a new building and conditions at the school improve dramatically.
After eight years of school Jane decides to leave, like her friend and confidante Miss Temple. She advertises her services as a governess, and receives one reply. It is from Alice Fairfax, who is a keeper of Thornfield Hall. She takes the position, teaching Adele Varens, a young French girl. While Jane is walking one night to a nearby town, a horseman passes her. The horse slips on ice and throws the rider. She helps him. Later, back at the mansion she learns that this man is Edward Rochester, master of the house. He wonders whether she bewitched his horse to make him fall. Adele is his ward, who could be his daughter; she was left in Mr. Rochester's care when her alleged mother was found with a rival of Mr. Rochester, laughing at his faults. Mr. Rochester denies he is her father and disowns her. Mr. Rochester and Jane enjoy each other's company and spend many hours together, and Jane longs for him.
Odd things happen at the house, such as a strange laugh, a mysterious fire in Mr. Rochester's room, on which Jane throws water, and an attack on Rochester's house guest, Mr. Mason. Jane hears that her aunt was calling for her, after being in much grief because her son has died. She returns to Gateshead and remains there for a month caring for her dying aunt. Mrs. Reed gives Jane a letter from Jane's uncle, asking for her to live with him. Mrs. Reed admits to telling her uncle that Jane had died of fever. Soon after, Jane's aunt dies, and Jane returns to Thornfield.